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F90655309
DeconvolutionLab2_Course_Bigradient.java
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Sun, Nov 3, 15:10
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R2075 deconvolution
DeconvolutionLab2_Course_Bigradient.java
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/*
* DeconvolutionLab2
*
* Conditions of use: You are free to use this software for research or
* educational purposes. In addition, we expect you to include adequate
* citations and acknowledgments whenever you present or publish results that
* are based on it.
*
* Reference: DeconvolutionLab2: An Open-Source Software for Deconvolution
* Microscopy D. Sage, L. Donati, F. Soulez, D. Fortun, G. Schmit, A. Seitz,
* R. Guiet, C. Vonesch, M Unser, Methods of Elsevier, 2017.
*/
/*
* Copyright 2010-2017 Biomedical Imaging Group at the EPFL.
*
* This file is part of DeconvolutionLab2 (DL2).
*
* DL2 is free software: you can redistribute it and/or modify it under the
* terms of the GNU General Public License as published by the Free Software
* Foundation, either version 3 of the License, or (at your option) any later
* version.
*
* DL2 is distributed in the hope that it will be useful, but WITHOUT ANY
* WARRANTY; without even the implied warranty of MERCHANTABILITY or FITNESS FOR
* A PARTICULAR PURPOSE. See the GNU General Public License for more details.
*
* You should have received a copy of the GNU General Public License along with
* DL2. If not, see <http://www.gnu.org/licenses/>.
*/
package
course
;
import
java.io.File
;
import
javax.swing.filechooser.FileSystemView
;
import
deconvolution.Deconvolution
;
import
ij.plugin.PlugIn
;
public
class
DeconvolutionLab2_Course_Bigradient
implements
PlugIn
{
private
String
desktop
=
FileSystemView
.
getFileSystemView
().
getHomeDirectory
().
getAbsolutePath
()
+
File
.
separator
+
"Desktop"
;
private
String
root
=
desktop
+
File
.
separator
+
"Deconvolution"
+
File
.
separator
;
private
String
res
=
root
+
"results"
+
File
.
separator
+
"bigradient"
+
File
.
separator
;
private
String
data
=
root
+
"data"
+
File
.
separator
+
"bigradient"
+
File
.
separator
;
public
DeconvolutionLab2_Course_Bigradient
()
{
new
File
(
res
).
mkdir
();
System
.
setProperty
(
"user.dir"
,
res
);
new
File
(
res
+
"TRIF"
).
mkdir
();
new
File
(
res
+
"RIF"
).
mkdir
();
new
File
(
res
+
"LW"
).
mkdir
();
new
File
(
res
+
"LW-ITER"
).
mkdir
();
new
File
(
res
+
"LW+"
).
mkdir
();
new
File
(
res
+
"LW+-ITER"
).
mkdir
();
new
File
(
res
+
"RL"
).
mkdir
();
new
File
(
res
+
"RL-ITER"
).
mkdir
();
new
File
(
res
+
"RLTV"
).
mkdir
();
new
File
(
res
+
"RLTV-ITER"
).
mkdir
();
new
File
(
res
+
"FISTA"
).
mkdir
();
new
File
(
res
+
"FISTA-ITER"
).
mkdir
();
String
psf
=
" -psf file "
+
data
+
"psf.tif -reference "
+
data
+
"ref.tif "
;
String
noisy
=
" -image file convnoise.tif"
;
new
Deconvolution
(
"run"
,
"-image file "
+
data
+
"ref.tif"
+
psf
+
" -algorithm SIM 0 1 1 -out stack convnoise -out stack conbnoise_8 rescaled byte noshow"
).
deconvolve
();
new
Deconvolution
(
"run"
,
noisy
+
psf
+
" -algorithm NIF -out stack NIF"
).
deconvolve
();
new
Deconvolution
(
"run"
,
noisy
+
psf
+
" -algorithm DIV -out stack DIV"
).
deconvolve
();
for
(
int
i
=
0
;
i
<=
3
;
i
++)
{
double
p
=
Math
.
pow
(
5
,
i
-
10
);
String
name
=
"RIF"
+
String
.
format
(
"%02d"
,
i
);
new
Deconvolution
(
"run"
,
noisy
+
psf
+
" -algorithm RIF "
+
p
+
out
(
"RIF"
+
File
.
separator
,
name
)).
deconvolve
();
}
for
(
int
i
=
0
;
i
<=
3
;
i
++)
{
double
p
=
Math
.
pow
(
5
,
i
-
10
);
String
name
=
"TRIF"
+
String
.
format
(
"%02d"
,
i
);
new
Deconvolution
(
"run"
,
noisy
+
psf
+
" -algorithm TRIF "
+
p
+
out
(
"TRIF"
+
File
.
separator
,
name
)).
deconvolve
();
}
String
lw
=
" -algorithm LW 20 1 -out mip @2 LW-ITER/I -out stats @1 LW nosave"
;
new
Deconvolution
(
"run"
,
noisy
+
psf
+
lw
).
deconvolve
();
new
File
(
res
+
"LW-ITER/I.tif"
).
delete
();
String
lwp
=
" -algorithm LW+ 20 1 -out mip @2 LW+-ITER/I -out stats @1 LW+ nosave"
;
new
Deconvolution
(
"run"
,
noisy
+
psf
+
lwp
).
deconvolve
();
new
File
(
res
+
"LW+-ITER/I.tif"
).
delete
();
String
rl
=
" -algorithm RL 20 -out mip @2 RL-ITER/I -out stats @1 RL nosave"
;
new
Deconvolution
(
"run"
,
noisy
+
psf
+
rl
).
deconvolve
();
new
File
(
res
+
"RL-ITER/I.tif"
).
delete
();
String
rltv
=
" -algorithm RLTV 20 10 -out mip @2 RLTV-ITER/I -out stats @1 RLTV nosave"
;
new
Deconvolution
(
"run"
,
noisy
+
psf
+
rltv
).
deconvolve
();
new
File
(
res
+
"RLTV-ITER/I.tif"
).
delete
();
String
fista
=
" -algorithm FISTA 20 1 1 Spline3 3 -mip @2 FISTA-ITER/I -out stats @1 FISTA nosave"
;
new
Deconvolution
(
"run"
,
noisy
+
psf
+
fista
).
deconvolve
();
new
File
(
res
+
"FISTA-ITER/I.tif"
).
delete
();
}
private
static
String
out
(
String
root
,
String
name
)
{
return
"out stats "
+
root
+
name
+
" -out stack "
+
root
+
name
+
"_32 -out stack "
+
root
+
name
+
"_8 rescaled byte noshow"
;
}
public
static
void
main
(
String
arg
[])
{
new
DeconvolutionLab2_Course_Bigradient
();
}
@Override
public
void
run
(
String
arg
)
{
new
DeconvolutionLab2_Course_Bigradient
();
}
}
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