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TraceInformation.py
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Sat, Jul 5, 15:07

TraceInformation.py
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import pdb
import numpy as np
import matplotlib.pyplot as plt
"""
This file was not coded by me (Colas Droin) but by Eric Paquet, and is therefore
not thoroughly commented, as I'm not sure about the meaning of each variable.
"""
class TraceInformation:
"""
This class is used to store and compute some information about the
experimental traces. Each trace is then enclosed in an instance of this
class.
"""
def __init__(self, hmm_area_signal_s, orig_idx, ind_s, raw_area, raw_signal,
peaks, cell_cycle_start, mitosis_start, serum, temp):
"""
Constructor of TraceInformation.
Parameters
----------
hmm_area_signal_s : dictionnary
todo.
orig_idx : integer
todo.
ind_s : integer
todo.
raw_area : list
Normalized experimental signal for the cell-cycle.
raw_signal : list
Normalized experimental signal for the circadian clock.
peaks : list
Indexes of the circadian peaks.
cell_cycle_start : list
Indexes of the first point after mitosis.
mitosis_start : list
Indexes of the beginning of the mitosis.
serum : string
Serum condition.
temp : int
Temperature condition.
"""
# size checks
expected_size = len(hmm_area_signal_s['area'])
assert(expected_size == \
len(hmm_area_signal_s['area_hmm']['mean']['theta']))
assert(expected_size == \
len(hmm_area_signal_s['area_hmm']['max']['theta']))
assert(expected_size == len(hmm_area_signal_s['signal']))
# Process the raw signal
raw_area = [val for val, ind in zip(raw_area,ind_s) if ind!=0]
raw_signal = [val for val, ind in zip(raw_signal,ind_s) if ind!=0]
cell_cycle_start = [val for val, ind in zip(cell_cycle_start,ind_s) \
if ind!=0]
mitosis_start = [val for val, ind in zip(mitosis_start,ind_s) if ind!=0]
peaks = [val for val,ind in zip(peaks, ind_s) if ind!=0]
# Just need to test one since they all use ind_s
assert(expected_size ==len(raw_area))
self.hmm_area_signal = hmm_area_signal_s
self.hmm_area_mean = hmm_area_signal_s['area_hmm']['mean']['theta']
self.hmm_area_max = hmm_area_signal_s['area_hmm']['max']['theta']
self.hmm_circa_mean = hmm_area_signal_s['circadian_hmm']['mean']['theta']
self.hmm_circa_max = hmm_area_signal_s['circadian_hmm']['max']['theta']
self.hmm_area_logP = hmm_area_signal_s['area_hmm']['logP']
self.hmm_circa_logP = hmm_area_signal_s['circadian_hmm']['logP']
self.raw_area = np.array(raw_area)
self.raw_circa = np.array(raw_signal)
self.normalized_area = hmm_area_signal_s['area']
self.normalized_circa = hmm_area_signal_s['signal']
self.cell_cycle_start = np.array(cell_cycle_start)
self.mitosis_start = np.array(mitosis_start)
self.peaks = np.array(peaks)
# Original indices in the raw data generated from matlab ie the .mat
#files
# IMPORTANT!!! this is a 1-base indice need to -1 to be use in python
self.orig_idx = orig_idx
self.temp = temp
self.serum = serum
def getNumPeaks(self):
"""
Get the number of circadian peaks of the current trace.
Returns
-------
The number of circadian peaks of the current trace.
"""
return sum(self.peaks)
def getNumDivs(self):
"""
Get the number of divisons of the current trace.
Returns
-------
The number of divisons of the current trace.
"""
return sum(self.cell_cycle_start)
def validAreaHMM(self):
"""
Check that the cell-cycle trace has been correctly annotated.
Returns
-------
A boolean indicating if the cell-cycle trace has been correctly
annotated.
"""
# just be sure the annotated mitosis start/cell cycle start
# have 1-0 values
is_valid = True
cc_idx = [ind for ind,val in zip(range(len(self.cell_cycle_start)),
self.cell_cycle_start) if val == 1]
look_around = 3
for ci in cc_idx:
cur_hmm = self.hmm_area_mean[ max(0,ci-look_around) : \
min(len(self.hmm_area_mean),ci+look_around+1)]
if (cur_hmm > 0.1).all():
is_valid = False
break
# cc_idx = [ind for ind,val in zip(range(len(self.cell_cycle_start)),
# self.hmm_area_mean) if 0 <= val <= 0.05]
# for ci in cc_idx:
# cur_hmm = np.array(self.cell_cycle_start[max(0,ci-look_around) : \
# min(len(self.hmm_area_mean),ci+look_around+1)])
# if (cur_hmm < 1).all():
# is_valid = False
# break
return is_valid
def validCircaHMM(self):
"""
Check that the circadian trace has been correctly annotated.
Returns
-------
A boolean indicating if the circadian trace has been correctly
annotated.
"""
is_valid = True
cc_idx = [ind for ind, val in zip(range(len(self.peaks)),self.peaks) \
if val == 1]
look_around = 3
for ci in cc_idx:
cur_hmm = self.hmm_circa_mean[max(0, ci-look_around) : \
min(len(self.peaks), ci+look_around+1)]
if (cur_hmm > 0.1).all():
is_valid = False
break
# cc_idx = [ind for ind,val in zip(range(len(self.cell_cycle_start)),
# self.hmm_circa_mean) if 0 <= val <= 0.05]
# for ci in cc_idx:
# cur_hmm = np.array(self.peaks[max(0, ci-look_around) : \
# min(len(self.hmm_area_mean),ci+look_around+1)])
# if (cur_hmm < 1).all():
# is_valid = False
# break
return is_valid
def __len__(self):
"""
Redefine the length function for the current class.
Returns
-------
The length of the circadian trace.
"""
return len(self.raw_circa)
def getEvents(self,n):
"""
Store in a string the cell-cycle events of the trace.
Parameters
----------
n : int
###TO CHECK
Number of points to ignore around mitosis ?
Returns
-------
The string of events.
"""
assert(n > 1)
events = ["" for i in range(len(self))]
for i in range(len(self)):
if self.cell_cycle_start[i] == 1:
events[i] += "C"
if self.mitosis_start[i] == 1:
events[i] += "M"
temp = []
for i in range(len(self)):
if events[i] != "":
temp.append([i,events[i]])
to_ret = []
if len(temp) > n:
for i in range(len(temp) - n + 1):
to_ret.append({'idx':[temp[i][0], temp[i+n-1][0]],
'tag':"".join(list(map(lambda a:a[1],temp[i:(i+n)])))})
return to_ret
def plotCircadian(self,dt=0.5,assignment='mean'):
"""
Plot the circadian trace.
Parameters
----------
dt : float
Time resolution
assignment : string
todo
"""
circadian_part = self.hmm_area_signal['circadian_hmm'][assignment]
plt.figure()
plt.plot(circadian_part['tspan'], circadian_part['model'],
label='P%s[model]' % assignment)
plt.plot(circadian_part['tspan'], self.normalized_circa, label='Signal')
plt.xlabel('t')
plt.plot(circadian_part['tspan'], circadian_part['theta'], '--',
label = r'$\theta$')
plt.plot(circadian_part['tspan'], circadian_part['amplitude'], '--',
label = "Amplitude")
dm_tmp = [ind for ind,val in zip(range(len(self)),
self.cell_cycle_start) if val == 1]
for cci in dm_tmp:
plt.plot([circadian_part['tspan'][cci],
circadian_part['tspan'][cci]], [0,1], 'c--')
dm_tmp_2 = [ind for ind,val in zip(range(len(self)),
self.peaks) if val == 1]
for cci in dm_tmp_2:
plt.plot([circadian_part['tspan'][cci],
circadian_part['tspan'][cci]],[0,1],'m--')
plt.plot([0,circadian_part['tspan'][-1]],[0,0],'k-')
plt.legend()
def plotArea(self,dt=0.5,assignment='mean'):
"""
Plot the cell-cycle trace.
Parameters
----------
dt : float
Time resolution
assignment : string
todo
"""
area_part = self.hmm_area_signal['area_hmm'][assignment]
plt.figure()
plt.plot(area_part['tspan'], area_part['model'],
label='P%s[model]' % assignment)
plt.plot(area_part['tspan'], self.normalized_area, label='Area')
plt.xlabel('t')
plt.plot(area_part['tspan'], area_part['theta'], '--',
label = r'$\theta$')
plt.plot(area_part['tspan'], area_part['amplitude'], '--',
label = "Amplitude")
for cci in [ind for ind,val in zip(range(len(self)),
self.cell_cycle_start) if val == 1]:
plt.plot([area_part['tspan'][cci], area_part['tspan'][cci]],
[0,1], 'c--')
for cci in [ind for ind,val in zip(range(len(self)),
self.peaks) if val == 1]:
plt.plot([area_part['tspan'][cci], area_part['tspan'][cci]],
[0,1], 'm--')
plt.plot([0,area_part['tspan'][-1]],[0,0],'k-')
plt.legend()

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