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scATAC-seq/scripts/10xgenomics_PBMC_5k_peaks_classification_7/classification_peaks.sh
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classification_peaks.sh
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# paths
## dir
data_dir_p="data/10xgenomics_PBMC_5k_peaks"
data_dir="data/10xgenomics_PBMC_5k"
pwm_dir="data/pwm/jaspar_2018_clustering/"
hg19_dir="data/genomes"
results_dir="results/10xgenomics_PBMC_5k_peaks_classification_7"
## matrix files
file_mat_open=$data_dir_p/'peaks_rmsk_openchromatin_1kb_read_atac.mat'
file_mat_nucl=$data_dir_p/'peaks_rmsk_nucleosomes_1kb_fragment_center.mat'
file_mat_seq=$data_dir_p/'peaks_rmsk_sequences_1kb.mat'
## file with seeds
file_seed=$results_dir'/peaks_rmsk_seed.txt'
mkdir -p $results_dir
touch $file_seed
# EM param
n_iter='1'
n_shift='971'
n_core=24
## PWM files
jun="$pwm_dir/cluster_3_node_23_20_motifs_prob.mat"
hif1a="$pwm_dir/cluster_4_node_31_3_motifs_prob.mat"
myc="$pwm_dir/cluster_4_node_22_4_motifs_prob.mat"
pu1="$pwm_dir/cluster_7_node_13_2_motifs_prob.mat"
cebpb="$pwm_dir/cluster_5_node_20_5_motifs_prob.mat"
irf4="$pwm_dir/cluster_31_node_4_5_motifs_prob.mat"
irf2="$pwm_dir/cluster_31_node_5_2_motifs_prob.mat"
lhx3="$pwm_dir/cluster_1_node_74_2_motifs_prob.mat"
foxh1="$pwm_dir/cluster_66_1_motifs_prob.mat"
sox3="$pwm_dir/cluster_33_node_1_2_motifs_prob.mat"
mef2c="$pwm_dir/cluster_20_4_motifs_prob.mat"
elf5="$pwm_dir/cluster_7_node_17_5_motifs_prob.mat"
stat6="$pwm_dir/cluster_32_node_STAT6_1_motifs_prob.mat"
nfe2="$pwm_dir/cluster_3_node_24_4_motifs_prob.mat"
ahr="$pwm_dir/cluster_4_node_30_2_motifs_prob.mat"
e2f2="$pwm_dir/cluster_39_node_1_2_motifs_prob.mat"
ctcf="$pwm_dir/cluster_48_node_ctcf_1_motifs_prob.mat"
klf="$pwm_dir/cluster_28_node_14_3_motifs_prob.mat"
nr4a1="$pwm_dir/cluster_2_node_12_4_motifs_prob.mat"
egr="$pwm_dir/cluster_28_node_13_4_motifs_prob.mat"
gata="$pwm_dir/cluster_21_node_5_6_motifs_prob.mat"
nfat="$pwm_dir/cluster_19_node_2_3_motifs_prob.mat"
runx="$pwm_dir/cluster_38_node_3_3_motifs_prob.mat"
# classify
for k in 23
do
## results files
file_prob=$results_dir/'peaks_rmsk_sequences_1kb_'$k'class_prob.mat4d'
file_mod1=$results_dir/'peaks_rmsk_openchromatin_1kb_read_atac_'$k'class_model.mat'
file_mod2=$results_dir/'peaks_rmsk_nucleosomes_1kb_fragment_center_'$k'class_model.mat'
file_mod3=$results_dir/'peaks_rmsk_sequences_1kb_'$k'class_model.mat'
seed=$(< /dev/urandom tr -dc _A-Z-a-z-0-9 | head -c${1:-15};echo)
echo "$file_prob $seed" >> $file_seed
bin/EMSequence --seq $file_mat_seq --class $k --motifs $jun,$hif1a,$myc,$pu1,$cebpb,$irf4,$irf2,$lhx3,$foxh1,$sox3,$mef2c,$elf5,$stat6,$nfe2,$ahr,$e2f2,$ctcf,$klf,$nr4a1,$egr,$gata,$nfat,$runx --shift $n_shift --flip --iter $n_iter --seed $seed --thread $n_core --out $file_prob
bin/ProbToModel --read $file_mat_open --prob $file_prob --thread $n_core 1> $file_mod1
bin/ProbToModel --read $file_mat_nucl --prob $file_prob --thread $n_core 1> $file_mod2
bin/ProbToModel --seq $file_mat_seq --prob $file_prob --thread $n_core 1> $file_mod3
# extend models
file_mod1_ext=$results_dir/'peaks_rmsk_openchromatin_1kb_read_atac_'$k'class_model_extended.mat'
file_mod2_ext=$results_dir/'peaks_rmsk_nucleosomes_1kb_fragment_center_'$k'class_model_extended.mat'
file_mod3_ext=$results_dir/'peaks_rmsk_sequences_1kb_'$k'class_model_extended.mat'
file_bed=$data_dir/'atac_v1_pbmc_5k_peaks_rmsk.bed'
file_fasta=$hg19_dir/'hg19.fasta'
file_bam_open=$data_dir/'atac_v1_pbmc_5k_possorted_filtered_30-84bp.bam'
file_bai_open=$data_dir/'atac_v1_pbmc_5k_possorted_filtered_30-84bp.bam.bai'
file_bam_nucl=$data_dir/'atac_v1_pbmc_5k_possorted_filtered_nucleosomes.bam'
file_bai_nucl=$data_dir/'atac_v1_pbmc_5k_possorted_filtered_nucleosomes.bam.bai'
bin/ReadModelExtender --bed $file_bed --bam $file_bam_open --bai $file_bai_open --prob $file_prob --from -500 --to 500 --ext 1000 --binSize 1 --method 'read_atac' --thread $n_core > $file_mod1_ext
bin/ReadModelExtender --bed $file_bed --bam $file_bam_nucl --bai $file_bai_nucl --prob $file_prob --from -500 --to 500 --ext 1000 --binSize 1 --method 'fragment_center' --thread $n_core > $file_mod2_ext
bin/SequenceModelExtender --bed $file_bed --fasta $file_fasta --prob $file_prob --from -500 --to 500 --ext 1000 --thread $n_core > $file_mod3_ext
done
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