Some settings to inject enough water with the micro-pump under the 25x objective at 37c for 8 hours.
Add 300 microliter of distilled water on the objective
Add your sample ( Coverslide) on the water
Find your focus
1.On the software
Active under "Setting", and under "Serial Port (USB)" = COM17
and under "Pump amplitude for interval water flow" = 75 volts.
Active under "Experiment";
and under "Active water flow pump interval, every" = 20 minutes.
and under "Water time at each time port" = 9 sec.
and Active the Pump
2.On the controller the values should be ,
HRZ = 9
Vpp = 95
In SRS mode