(IMPORTANT)
Once you summon the DEVILS, you can't quantify your image anymore! This tool is made for visual inspection ONLY!
=What is DEVILS useful for?=
DEVIL is useful to create an image that shows features within several magnitudes of intensities.
DEVIL homogenizes the intensities and removes (global and local) background so you can display high and low intensities using one display scale.
===Top : Original {F1143652, size=full } Bottom : DEVIL ===
**Sample preparation **: Olivier Hagens, LNMC, EPFL
**Image acquisition ** : Thierry Larroche, BIOP, EPFL
**Image enhancement** : Romain Guiet, BIOP, EPFL
=What does DEVILS mean?=
DEVILS stands for Display Enhancement for Visual Inspection in Large Stack.
(WARNING)
This tool is made for visual inspection ONLY! NO measurement! NO Quantification! No p values nor some * at the end!
=What is DEVILS exactly?=
It's a workflow that combines several classic Image Processing tools to decrease the difference between high and low-intensity signals within an image.
=What is DEVILS based on?=
DEVILS is an ImageJ/Fiji script, using the ImageJ macro language or a plugin (see below Install).
DEVILS uses a combination of three plugins/functions of **ImageJ/Fiji**:
* Division by a blurred version of the image
* Square Root Calculation
* Subtract Background
=What are the DEVILS drawbacks? =
It increases the noise, by a significant amount !
So your images should use as much display range as possible (without saturation of course) to get a satisfying result.
=How to Install and Use DEVILS?=
As a [[ https://c4science.ch/w/bioimaging_and_optics_platform_biop/image-processing/imagej_tools/devil/ijp-devils/ | Plugin ]]
or
As a [[ https://c4science.ch/w/bioimaging_and_optics_platform_biop/image-processing/imagej_tools/devil/ijm-devil/ | Macro]] (no more support)