=Indivual Field of VIew=
The **uneven illumintation** make an homogenous Field of VIew (FOV) looks uneven.
As a consequence, the gray levels in the corner of the FOV can be as low as 50% of the maximum in the center.
{F5880611, size = full}
= When doing tilling =
{F5837562, size=full}
Please consider doing it with more zoom !
Indeed in you fixe the **Pixel Size**
| Microscope| LSM710 |
| Objective| 40X Oil NA 1.39|
| Zoom | 0.6 , 1, 1.5, 2|
| Pixel Dwell | 0.65 us (0.64-0.67) |
|Pixel Size | 0.18 micron|
| xy size of Tiling | 1500 micron |
It doesn't make take so much time to do it with a higher zoom !
|Zoom | 0.6 | 1 | 1.5 | 2 |
|Tile ... x ... | 5 | 8 | 11 | 15 |
|Ind. Tile Size (px) | 2000 | 1200 | 800 | 600 |
| Time (s) | 300 | 330 | 300 | 360 |
{F5837562, size=full}(because each individual tile is smaller ! )