Homogeneous field acquisition.
Zooming helps decreasing intensity range between corner (min) and center (max) of the image.
{F5837353, size =full}
= Example With Cells =
Acquired on LSM710: 3 channels, 1AU pinhole for each channel, pixel size 210nm/px and zoom 0.5.
First the cell in the corner was acquired, then the cell in the center, to show the trend goes beyond photobleaching.
{F5884771, size=full}
Values on the right represent rounded average intensity for the same cell at the bottom right of the field of view(top row) at the center of the field of view(bottom row)
The loss of intensity is represented by dimming the color of the boxes around the measurements.
== Loss ==
|Channel| Intensity Cell @ Edge| Intensity Cell @ Center | Loss |
|---------|--------------------------|-------------------------|------|
| Phalloidin| 4 | 28 | 85% |
| DAPI| 46 | 143 | 68% |
| Mitotracker | 20 | 60 | 67%|