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DEVILS
Updated 2,276 Days AgoPublic

Once you summon the DEVILS, you can't quantify your image anymore! This tool is made for visual inspection ONLY!

What is DEVILS useful for?

DEVILS is useful to create an image that shows features within several magnitudes of intensities.
DEVILS homogenizes the intensities and removes (global and local) background so you can display high and low intensities using one display scale.

Top : Original
Bottom : DEVIL

Sample preparation : Olivier Hagens, LNMC, EPFL
Image acquisition : Thierry Larroche, BIOP, EPFL
Image enhancement : Romain Guiet, BIOP, EPFL

What does DEVILS mean?

DEVILS stands for Display Enhancement for Visual Inspection in Large Stack.

This tool is made for visual inspection ONLY! NO measurement! NO Quantification! No p values nor some * at the end!

What is DEVILS exactly?

It's a workflow that combines several classic Image Processing tools to decrease the difference between high and low-intensity signals within an image.

What is DEVILS based on?

DEVILS is an ImageJ/Fiji script, using the ImageJ macro language or a plugin (see below Install).

DEVILS uses a combination of three plugins/functions of ImageJ/Fiji:

  • Division by a blurred version of the image
  • Square Root Calculation
  • Subtract Background

What are the DEVILS drawbacks?

It increases the noise, by a significant amount !
So your images should use as much display range as possible (without saturation of course) to get a satisfying result.

How to Install and Use DEVILS?

As a Plugin
or
As a Macro (no more support)

Last Author
romainGuiet
Last Edited
Sep 28 2018, 11:54

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oburri moved this document from Restricted Phriction Wiki DocumentApr 12 2018, 08:23
romainGuiet changed the visibility from "All Users" to "Public (No Login Required)".Apr 12 2018, 08:41
romainGuiet edited the content of this document. (Show Details)Apr 12 2018, 08:48
romainGuiet edited the content of this document. (Show Details)
romainGuiet edited the content of this document. (Show Details)Sep 28 2018, 11:54