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Tiling
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Indivual Field of VIew

Uneven illumintation makes an homogenous Field of VIew (FOV) appear uneven.
As a consequence, the gray levels in the corner of the FOV can be as low as 50% of the maximum in the center.

When doing tilling

MicroscopeObjectiveZoomPixel DwellPixel Sizexy size of Tiling
LSM71040X Oil NA 1.390.6 , 1, 1.5, 20.65 us (0.64-0.67)0.18 micron1500 micron

Please consider doing it with more zoom ! Indeed in you fixe the Pixel Size, it doesn't make take so much time to do it with a higher zoom !

Zoom0.611.52
Tile ... x ...581115
Ind. Tile Size (px)20001200800600
Time (s)300330300360
Intensity variation range (max - min)70%50%30%20 %

(because each individual tile is smaller ! )

Last Author
chiarutt
Last Edited
May 17 2019, 12:50

Event Timeline

romainGuiet created this document.Jan 19 2018, 16:04
romainGuiet edited the content of this document. (Show Details)
romainGuiet edited the content of this document. (Show Details)Jan 22 2018, 08:48
romainGuiet edited the content of this document. (Show Details)Jan 22 2018, 08:56
oburri edited the content of this document. (Show Details)Jan 22 2018, 09:13
oburri changed the visibility from "All Users" to "Public (No Login Required)".Dec 3 2018, 10:57
chiarutt changed the title from Tilling to Tiling.May 17 2019, 12:50
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