= Tutorial=
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[[ https://c4science.ch/w/bioimaging_and_optics_platform_biop/image-processing/imagej_tools/perkinelmer-stitching/ | Using Fiji to process your Operetta images]]
= Mind the Byte =
**One full well from a 96-well plate **(4.5mm x 4.5mm field of view)
Full resolution acquisition **20x air objective**
**1Gb per plane **(Single channel, single slice, single timepoint)
Example acquisition, 2 channels, 11 slices : **9.35 GB per well**
= Write archive =
It takes **~1 hour for 100 GB**
= Harmony 4.8 installation =
Start Autorun.exe
\\svfas6.epfl.ch\biop\biop\MICROSCOPY\Operetta\2019-10-17 SW-Update Harmony 4.9 MDe
Select: Office PC > install
Warning : if the computer states : "you need to restart the computer", restarting the software will not do anything...
To proceed with the installation :
* Run Regedit.exe
* Find the Windows Registry : HKEY_LOCAL_MACHINE\SYSTEM\CurrentControlSet\Control\Session Manager : PendingFileRnameOperations
* Delete the PendingFileRnameOperations registry (right click > delete > ok)
* You can now proceed to do the installation
The **Region and Language Settings of the PC should be in English UK or US** otherwise the software crashes when you start it.
Connect the Operetta database.
Start Harmony 4.9.
Go on: settings > data management > database settings
Add a new database (click on **+**)
Computer name: SV-01-366
Alias name: Operetta CLS
= Plate reference =
| **Brand**| **Ref Name** | **Ref Nbr** | **Qty** | **EUR**| **Remarque** |
|PerkinElmer | [[ http://www.perkinelmer.com/fr/product/cellcarrier-96-ultra-lid-8x20b-6055300 | CellCarrier Ultra 96 ]] | 6055300|50 | 388.00 | works with 20x et 63x water|
|PerkinElmer | [[ http://www.perkinelmer.com/fr/product/cellcarrier-96-ultra-lid-8x20b-6055308 | CellCarrier Ultra 96 ]] | 6055308 |160 |1241.00 |
|PerkinElmer | [[ http://www.perkinelmer.com/fr/product/viewplate-96-f-tc-10x5b-6005182 | ViewPlate-96F]] | 6005182 | 50 | 390.00 | Can't access the wells on the border with high mag. obj. + issue with finding focus using 40x & 63x |
|PerkinElmer | [[ http://www.perkinelmer.com/fr/product/viewplate-96-f-tc-10x5b-6005225 | ViewPlate-96F]] | 6005225 | 5x10 | 372.00 |
|Falcon | [[ https://ecatalog.corning.com/life-sciences/b2c/EUOther/en/Microplates/Assay-Microplates/96-Well-Microplates/Falcon%C2%AE-96-well-Polystyrene-Microplates/p/353219 | 96Well ImagingPlate ]] | FA-353219 | 4 x 8 | CHF 206-290 | Can't access the wells on the border with high mag. obj. + issue with finding focus using 63x |