Some settings to inject enough water with the micro-pump under the 25x objective at 37c for 8 hours. Add 300 microliter of distilled water on the objective Add your sample ( Coverslide) on the water Find your focus 1.On the software Active under "Setting", and under "Serial Port (USB)" = COM17 and under "Pump amplitude for interval water flow" = 75 volts. Active under "Experiment"; and under "Active water flow pump interval, every" = 20 minutes. and under "Water time at each time port" = 9 sec. and Active the Pump 2.On the controller the values should be , HRZ = 9 Vpp = 95 In SRS mode

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