Phriction Projects Wikis Bioimaging And Optics Platform Microscopy Micro-pump SP8 History Version 2 vs 3
Version 2 vs 3
Version 2 vs 3
Content Changes
Content Changes
Some settings to inject enough water with the micro-pump (SP8 FLIM) under the 25x IMM objective at 37c for 8 hours.
1. Setup our sample with the 25x IMM objectif
Add 300 microliter of distilled water on the objective
Add your sample ( Coverslide) on the water
Find your focus
2.On the software "AOI Controller"
Active under "Setting", and under "Serial Port (USB)" = COM17
and under "Pump amplitude for interval water flow" = 75 volts.
Active under "Experiment";
and under "Active water flow pump interval, every" = 20 minutes.
and under "Water time at each time port" = 9 sec.
and Active the Pump
3.On the controller the values should be ,
HRZ = 9
Vpp = 95
In SRS mode
Settings to inject enough water with the micro-pump (SP8-FLIM) under the 25x IMM objective at 37c.
These settings have been tested for 8 hours.
# Switch ON the pump (button at the back)
# Start the software "AOI Controller"
- Tab "Settings", select "Serial Port (USB)" = COM8 (or check what's written on the green tape on top of the controller)
- Tab "Settings" select "Pump amplitude for interval water flow" = 75 volts
- Tab "Experiment":
- Check "Activate water flow (pump interval) every"
- "Active water flow pump interval, every" = 20 minutes.
- "Water time at each time port" = 9 sec.
- You can start the pump whenever the experiment is ready with the button "Start Pump"
# On the controller the values should be:
- HRZ = 9
- Vpp = 95
- In SRS mode
- You can change these values with the switches on the controller
# Setup your sample with the 25x IMM objectif
- Check that there is distilled water in the water reservoir
- Initiate the flow with the pump until you see water flowing out of the objective
- Add 300 uL of distilled water on the objective
- Add your sample (Coverslip) on the water
- Find your focus
- You can start the pump whenever the experiment is ready with the button "Start Pump"
- Check again the settings displayed on the water pump controller
Some settings to inject enough water with the micro-pump (SP8 SP8-FLIM) under the 25x IMM objective at 37c.
These settings have been tested for 8 hours.
1.# Switch ON the pump (button at the back)
# Start the software "AOI Controller"
- Tab "Settings", Setup our sample with the 25x IMM objectifselect "Serial Port (USB)" = COM8 (or check what's written on the green tape on top of the controller)
- Tab "Settings" select "Pump amplitude for interval water flow" = 75 volts
Add 300 microliter of distilled water on the objective - Tab "Experiment":
Add your sample ( Coverslide) on the water - Check "Activate water flow (pump interval) every"
Find your focus
2.On the software "AOI Controller" - "Active water flow pump interval, every" = 20 minutes.
- "Water time at each time port" = 9 sec.
Active under "Setting", and under "Serial Port (USB)" = COM17 - You can start the pump whenever the experiment is ready with the button "Start Pump"
and under "Pump amplitude for interval water flow" = 75 volts.
Active under "Experiment";# On the controller the values should be:
- HRZ = 9
and under "Active water flow pump interval, every" = 20 minutes. - Vpp = 95
- In SRS mode
- You can change these values with the switches on the controller
# Setup your sample with the 25x IMM objectif
- Check that there is distilled water in the water reservoir
and under "Water time at each time port" = 9 sec. - Initiate the flow with the pump until you see water flowing out of the objective
and Active the Pump
3.On the controller the values should be ,
HRZ = 9
Vpp = 95 - Add 300 uL of distilled water on the objective
- Add your sample (Coverslip) on the water
- Find your focus
In SRS mode - You can start the pump whenever the experiment is ready with the button "Start Pump"
- Check again the settings displayed on the water pump controller
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