=Indivual Field of VIew= The **uneven illumintation** make an homogenous Field of VIew (FOV) looks uneven. As a consequence, the gray levels in the corner of the FOV can be as low as 50% of the maximum in the center. {F5880611, size = full} = When doing tilling = {F5837562, size=full} Please consider doing it with more zoom ! Indeed in you fixe the **Pixel Size** | Microscope| LSM710 | | Objective| 40X Oil NA 1.39| | Zoom | 0.6 , 1, 1.5, 2| | Pixel Dwell | 0.65 us (0.64-0.67) | |Pixel Size | 0.18 micron| | xy size of Tiling | 1500 micron | It doesn't make take so much time to do it with a higher zoom ! |Zoom | 0.6 | 1 | 1.5 | 2 | |Tile ... x ... | 5 | 8 | 11 | 15 | |Ind. Tile Size (px) | 2000 | 1200 | 800 | 600 | | Time (s) | 300 | 330 | 300 | 360 | (because each individual tile is smaller ! )

=Indivual Field of VIew= **Uneven illumintation** makes an homogenous Field of VIew (FOV) appear uneven. As a consequence, the gray levels in the corner of the FOV can be as low as 50% of the maximum in the center. {F5880611, size = full} = When doing tilling = {F5837562, size=full} | Microscope| Objective| Zoom |Pixel Dwell | Pixel Size | xy size of Tiling | | LSM710 | 40X Oil NA 1.39| 0.6 , 1, 1.5, 2| 0.65 us (0.64-0.67) |0.18 micron| 1500 micron | Please consider doing it with more zoom ! Indeed in you fixe the **Pixel Size**, it doesn't make take so much time to do it with a higher zoom ! |Zoom | 0.6 | 1 | 1.5 | 2 | | -- | -- | -- | --| -- | |Tile ... x ... | 5 | 8 | 11 | 15 | |Ind. Tile Size (px) | 2000 | 1200 | 800 | 600 | | Time (s) | 300 | 330 | 300 | 360 | | Intensity variation range (max - min) | 70% | 50% | 30% | 20 % | (because each individual tile is smaller ! )

=Indivual Field of VIew= The **u**Uneven illumintation** makes an homogenous Field of VIew (FOV) looksappear uneven. As a consequence, the gray levels in the corner of the FOV can be as low as 50% of the maximum in the center. {F5880611, size = full} = When doing tilling = {F5837562, size=full} Please consider doing it with more zoom ! Indeed in you fixe the **| Microscope| Objective| Zoom |Pixel Dwell | Pixel Size** | Microscope| LSM710 | | xy size of Tiling | | Objective| LSM710 | 40X Oil NA 1.39| | Zoom | 0| 0.6 , 1, 1.5, 2| | Pixel Dwell | 0.65 us (0.64-0.67) | |Pixel Size | 0.18 micron 0.65 us (0.64-0.67) |0.18 micron| 1500 micron | Please consider doing it with more zoom ! Indeed in you fixe the **Pixel Size**, it doesn't make take so much time to do it with a higher zoom ! |Zoom | 0.6 | 1 | 1.5 | 2 | | xy size of Tiling | 1500 micron | It doesn't make take so much time to do it with a higher zoom ! | -- | -- | -- | --| -- | |Zoom | 0.6 | 1 | 1.5|Tile ... x ... | 5 | 8 | 11 | 2 |15 | |Tile ... x ..|Ind. | 5 | 8 | 11Tile Size (px) | 2000 | 1200 | 800 | 15 |600 | |Ind. Tile Size (px| Time (s) | 20300 | 120330 | 8300 | 600 |360 | | Time (s| Intensity variation range (max - min) | 300 | 33070% | 50% | 300 | 360 |% | 20 % | (because each individual tile is smaller ! )