= Rule of Thumb = {F5928403, size=full} = Example = {F5928405, size=full} = Origin of the "Rule of Thumb"= {F5928589, size=full} = Limit of the "Rule of Thumb"= (IMPORTANT) If you're object size is below the limit of diffraction (see [[ https://en.wikipedia.org/wiki/Diffraction-limited_system | Abbe diffraction limit ]]) , ie roughly 250 nm, no need to make pixel size of 2 Å ! Please use this [[ https://svi.nl/NyquistCalculator | Nyquist calculator ]] to get the smallest size of pixel which is meaningful with your system.

= Rule of Thumb = {F5928403, size=full} = Example = {F5928405, size=full} = Limit = (IMPORTANT) If you're object size is below the limit of diffraction (see [[ https://en.wikipedia.org/wiki/Diffraction-limited_system | Abbe diffraction limit ]]) , ie roughly 250 nm, no need to make pixel size of 2 Å ! Please use this [[ https://svi.nl/NyquistCalculator | Nyquist calculator ]] to get the smallest size of pixel which is meaningful with your system. = Origin = {F5928589, size=full}

= Rule of Thumb = {F5928403, size=full} = Example = {F5928405, size=full} = Origin of the "Rule of Thumb"= {F5928589, size=full} == Limit of the "Rule of Thumb"=t = (IMPORTANT) If you're object size is below the limit of diffraction (see [[ https://en.wikipedia.org/wiki/Diffraction-limited_system | Abbe diffraction limit ]]) , ie roughly 250 nm, no need to make pixel size of 2 Å ! Please use this [[ https://svi.nl/NyquistCalculator | Nyquist calculator ]] to get the smallest size of pixel which is meaningful with your system. = Origin = {F5928589, size=full}