Phriction Projects Wikis Bioimaging And Optics Platform Microscopy PerkinElmer Operetta FCS History Version 2 vs 3
Version 2 vs 3
Version 2 vs 3
Edits
Edits
- Edit by romainGuiet, Version 3
- Feb 14 2018 13:55
- Edit by bozzo, Version 2
- Jan 29 2018 11:27
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Content Changes
Content Changes
= Mind the Byte =
**One full well from a 96-well plate **(4.5mm x 4.5mm field of view)
Full resolution acquisition **20x air objective**
**1Gb per plane **(Single channel, single slice, single timepoint)
Example acquisition, 2 channels, 11 slices : **9.35 GB per well**
= Harmony 4.6 installation =
Start the .exe
\\svfas6\biop\biop\MICROSCOPY\Operetta\Harmony 4.6 RC 265\ Startup.exe
Select: Office PC > install
The **Region and Language Settings of the PC should be in English UK or US** otherwise the software crashes when you start it.
Connect the Operetta database.
Start Harmony 4.6.
Go on: settings > data management > database settings
Add a new database (click on **+**)
Computer name: sv-01-366
Alias name: Operetta CLS
= Tutorial=
{F6440386}
= Mind the Byte =
**One full well from a 96-well plate **(4.5mm x 4.5mm field of view)
Full resolution acquisition **20x air objective**
**1Gb per plane **(Single channel, single slice, single timepoint)
Example acquisition, 2 channels, 11 slices : **9.35 GB per well**
= Harmony 4.6 installation =
Start the .exe
\\svfas6\biop\biop\MICROSCOPY\Operetta\Harmony 4.6 RC 265\ Startup.exe
Select: Office PC > install
The **Region and Language Settings of the PC should be in English UK or US** otherwise the software crashes when you start it.
Connect the Operetta database.
Start Harmony 4.6.
Go on: settings > data management > database settings
Add a new database (click on **+**)
Computer name: sv-01-366
Alias name: Operetta CLS
= Tutorial=
{F6440386}
= Mind the Byte =
**One full well from a 96-well plate **(4.5mm x 4.5mm field of view)
Full resolution acquisition **20x air objective**
**1Gb per plane **(Single channel, single slice, single timepoint)
Example acquisition, 2 channels, 11 slices : **9.35 GB per well**
= Harmony 4.6 installation =
Start the .exe
\\svfas6\biop\biop\MICROSCOPY\Operetta\Harmony 4.6 RC 265\ Startup.exe
Select: Office PC > install
The **Region and Language Settings of the PC should be in English UK or US** otherwise the software crashes when you start it.
Connect the Operetta database.
Start Harmony 4.6.
Go on: settings > data management > database settings
Add a new database (click on **+**)
Computer name: sv-01-366
Alias name: Operetta CLS
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