Resolution & Pixel Size

Updated 2,462 Days AgoPublic

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On a system with a camera the pixel size is function of the :

• Camera (what is the size of a pixel on the camera chip)
• Objective magnification
• Binning (to put adjacent pixels together , that makes pixel bigger, image has less pixels, but higher sensitivity)
• [presence of an additional lens in the light path ]

On a Point Scanning Confocal equipped with PMT (or fancier device) you scan one pixel at a time, one after another. The pixel size is function of the :

• Scanning Head Specification
• Objective magnification
• Zoom
• Number of pixels of your image

So on a defined microscope, for a defined Objective magnification and Zoom if you make an image with 256, 512 or 1024 pixels your Pixel Size will get smaller and smaller (0.2 um, 0.1 um, 0.05um per pixel).

The N.A. of the objective will affect your resolution power, so does the wavelength you use (see Abbe diffraction limit )